Articles & Publications

The investigation of hair porosity and the effect of cosmetic products on this parameter for claim substantiation has traditionally relied on indirect measurements. This is primarily because the techniques available for direct assessment require specialized and expensive equipment, making them unfeasible for routine commercial use. However, understanding and addressing hair porosity is critical for cosmetic companies, as it directly impacts the overall integrity of the hair fiber and influences consumer satisfaction. Porosity affects how well hair retains moisture and how it responds to treatments, which is crucial for the long-term performance of hair care products. Recently, with the advancement of image analysis software and plugins, new methods have emerged to more directly evaluate hair porosity. One promising approach is the use of cross-sectional images of hair fibers obtained through SEM (Scanning Electron Microscopy). This method allows to measure the internal porosity of the hair fiber, offering a more direct and precise analysis. However, the main challenge lies in obtaining high-quality transverse cuts of the hair that preserve the internal structure for accurate measurements. Given these developments, the goal of this study is to establish a reliable, cost-effective methodology for measuring the number and size of pores in hair fibers. The proposed methodology involves obtaining precise hair cross-sections using advanced histological techniques, followed by image analysis through specialized plugins to count and measure the pores. The development process was divided into two phases. First, we investigated the most effective combination of embedding medium and cutting tools to produce cross-sections that maintain the hair’s internal morphology. In the second phase, the methodology was validated for reproducibility and its ability to differentiate between various cosmetic treatments.

Libbs Farmacêutica Ltda, São Paulo/SP – Brazil Kosmoscience Group, Valinhos/SP – Brazil Conflict of interest - Ana Lucia Tabarini Alves Pinheiro, Vania Renata Gonçalves, Barbara de Freitas Carli, Gustavo Facchini, Janayna de Godoi Rampazo, Samara Eberlin and Juliana Rodrigues Pinto, are employees at Kosmoscience Group, responsible for carrying out the study. - Paula Bonilha Fernandes, Vivienne Carduz Castilho and Maura Gonzaga Lapa are employees at Libbs Pharmaceutical, study sponsor. Objective 1: to assess the safety and effectiveness of a skincare soap in controlling the clinical symptoms of atopic dermatitis, using subjective evaluation techniques. Objective 2: to investigate the impact of the skincare soap on microbiota modulation by analyzing gene expression of Staphylococcus aureus and Staphylococcus epidermidis in swabs collected during the clinical trial. Atopic dermatitis (AD) is associated with an overcolonization of Staphylococcus aureus, especially during flares, resulting in dysbiosis and decreased bacterial diversity. This study was based on the hypothesis that home use of skincare soap provides gentle cleansing without harming the skin in children with skin prone to AD. Skincare soap (UmAI) was formulated with a blend of lipids, glycine soy and bisabolol. Over 28 days, pediatric research participants with a diagnosis or history of non-active atopic dermatitis, or a first-degree family history (n=34; 1-11y) were instructed to apply the soap to damp skin on the face and body during each bath (Ethics Committee nº5.392.397). Cutaneous and ophthalmological acceptability was evaluated before (D0) and after 28 days (D28) using UmAI, and no signs of irritation, allergic reactions, or discomfort were related. Perceived effectiveness was answered by the participant or their parents on D28. Results indicated that soap gently cleanses the skin without causing damage or dryness, and 85% of participants reported a sensation of softness and hydration. Microbiota assessment involved quantifying Staphylococcus aureus and Staphylococcus epidermidis in forearm swab samples using Real-Time qPCR on D0 and D28. Compared to D0, UmAI significantly reduced Staphylococcus aureus up to 80% of the panel, and increased Staphylococcus epidermidis up to 60%. In conclusion, these findings support the inclusion of UmAI skincare soap in the daily hygiene routine for children with AD-prone, emphasizing its mild cleansing properties. The effectiveness of UmAI is likely linked to its ability to balance the dysbiosis commonly associated with AD. References:Koh LF, Ong RY, Common JE. Skin microbiome of atopic dermatitis. Allergol Int. 2022 Jan;71(1):31-39. doi: 10.1016/j.alit.2021.11.001. Epub 2021 Nov 24. PMID: 34838450. Byrd AL, Deming C, Cassidy SKB, Harrison OJ, Ng WI, Conlan S; NISC Comparative Sequencing Program; Belkaid Y, Segre JA, Kong HH. Staphylococcus aureus and Staphylococcus epidermidis strain diversity underlying pediatric atopic dermatitis. Sci Transl Med. 2017 Jul 5;9(397):eaal4651. doi: 10.1126/scitranslmed.aal4651. PMID: 28679656; PMCID: PMC5706545. Demessant-Flavigny AL, Connétable S, Kerob D, Moreau M, Aguilar L, Wollenberg A. Skin microbiome dysbiosis and the role of Staphylococcus aureus in atopic dermatitis in adults and children: A narrative review. J Eur Acad Dermatol Venereol. 2023 Jun;37 Suppl 5:3-17. doi: 10.1111/jdv.19125. PMID: 37232427. van den Bogaard EH, Elias PM, Goleva E, Berdyshev E, Smits JPH, Danby SG, Cork MJ, Leung DYM. Targeting Skin Barrier Function in Atopic Dermatitis. J Allergy Clin Immunol Pract. 2023 May;11(5):1335-1346. doi: 10.1016/j.jaip.2023.02.005. Epub 2023 Feb 19. PMID: 36805053; PMCID: PMC11346348. Sangaphunchai P, Kritsanaviparkporn C, Treesirichod A. Association Between Staphylococcus Aureus Colonization and Pediatric Atopic Dermatitis: A Systematic Review and Meta-Analysis. Indian J Dermatol. 2023 Nov-Dec;68(6):619-627. doi: 10.4103/ijd.ijd_453_22. Epub 2024 Jan 9. PMID: 38371569; PMCID: PMC10868995. Paller AS, Kong HH, Seed P, Naik S, Scharschmidt TC, Gallo RL, Luger T, Irvine AD. The microbiome in patients with atopic dermatitis. J Allergy Clin Immunol. 2019 Jan;143(1):26-35. doi: 10.1016/j.jaci.2018.11.015. Epub 2018 Nov 23. Erratum in: J Allergy Clin Immunol. 2019 Apr;143(4):1660. doi: 10.1016/j.jaci.2019.01.022. PMID: 30476499; PMCID: PMC7163929. Geoghegan JA, Irvine AD, Foster TJ. Staphylococcus aureus and Atopic Dermatitis: A Complex and Evolving Relationship. Trends Microbiol. 2018 Jun;26(6):484-497. doi: 10.1016/j.tim.2017.11.008. Epub 2017 Dec 9. PMID: 29233606.

Introduction Skin aging involves intrinsic and extrinsic factors, leading to epidermal thinning, loss of dermal ridges, and attenuation of papillae, which impair epidermal nourishment.1,2 Additionally, collagen and elastin degradation in the dermis, compounded by reduced fibroblast activity, compromises skin structure and resilience.3,4 This study aimed to clinically evaluate the efficacy of the skincare formulation F4565.33896 in reducing signs of skin aging, using biometric and imaging analyses, along with immunofluorescence analysis of skin biopsies to assess biological markers related to aging. Methods This study, approved by the Ethics Committee (project 63574322.4.0000.5514), included 33 participants (64±3 years). A formulation (F4565.33896), containing tocopherol, hydrolyzed hyaluronic acid, and plant-derived bioactive, was applied to one forearm and the face for 56 days, with the opposite forearm as a control. Evaluations included cutometric measurements for firmness and elasticity, digital and 3D imaging for wrinkles, texture, and contours, ultrasonography for dermal density (T0, T28, T56), and skin biopsies (T0, T28) for biomarker analysis (type I pro-collagen, elastin, hyaluronic acid). Results Treatment with a formulation F4565.33896 significantly improved skin firmness (19.7% at T28, 20.9% at T56) and elasticity (12.7% at T28, 13.4% at T56). Wrinkles and fine lines reduced by up to 13.7% at T28 and 16.2% at T56, while deep wrinkles decreased by up to 6.9% and 8.5%. Facial texture improved by 8.1% at T28 and 9.1% at T56, and sagging reduced with enhanced contour definition (1.1% at T28, 1.4% at T56). Dermal density increased significantly (11.8% at T28, 19.5% at T56). Additionally, marked boosts in type I pro-collagen (191.85%), elastin (283.95%), and hyaluronic acid production (112.58%) was observed at T28. Discussion and Conclusion This study, employing advanced evaluation methods, demonstrated that F4565.33896 significantly improved skin firmness and elasticity, reduced wrinkles and sagging, while also increasing dermal density and enhancing the synthesis of type I collagen, elastin, and hyaluronic acid, suggesting extracellular matrix regeneration. These findings highlight the potential of the active ingredients in the formulation to improve the biomechanical properties of the skin and mitigate signs of aging, consistent with the observed clinical results. Keyword: anti-aging, aging signs evaluation, skin texture

Resumen: La formación de burbujas en el cabello, llamada “Bubble Hair”, es un fenómeno que ocurre principalmente debido al sobrecalentamiento de la fibra capilar, especialmente cuando el cabello está húmedo. Cuando el agua se convierte en vapor, se expande y, al estar “atrapada” dentro de la fibra capilar, no puede escapar fácilmente. Esta presión interna causa la formación de pequeñas burbujas a lo largo del cabello, las cuales generan áreas debilitadas, dejando el cabello más susceptible a daños y quiebres. Dentro de este contexto, el objetivo de este trabajo es evaluar la eficacia de productos cosméticos en la reparación de burbujas formadas en el interior de la fibra capilar. Para esto, se recreó la condición de Bubble Hair en el laboratorio, aplicando una plancha sobre fibras de cabello húmedas. Luego, los cabellos fueron tratados con los productos cosméticos a ser estudiados, manteniendo un grupo de control sin tratamiento para efectos de comparación. La morfología de los cabellos fue analizada mediante microscopía óptica para observar las alteraciones estructurales visibles, mientras que la resistencia mecánica fue evaluada mediante ensayos de tracción para verificar la recuperación de la fuerza del cabello. Además, un grupo adicional de mechones también sometidos a la condición de Bubble Hair fue tratado con los mismos productos y evaluado mediante espectroscopia de infrarrojo FTIR-ATR. Este método permite verificar la integridad de la fibra capilar al identificar posibles cambios en la conformación de la proteína del cabello. Los resultados mostraron una mejora en la condición de Bubble Hair después de la aplicación de los productos cosméticos probados, reduciendo visiblemente las burbujas formadas en el interior del cabello.

IFSCC 2025 Congress 2025, Full Paper ( Abstract Reference Number) IFSCC 2025 Congress 2025, Full Paper ( Abstract Reference Number) 2 of 8 whether the strands floated on the surface or sank. Based on this observation, they classified the strands as: Low porosity for those that floated; Normal porosity for those in which part of the strand floated and part sank; High porosity for those in which all strands sank [1]. This type of indirect measurement is used by consumers because it is easy to perform and does not require expensive measuring instruments, however it does not provide numerical measurements of porosity or information about the shape of the pores, that are important data in the study of the interaction between the hair strand and cosmetic products. However, advancements in image analysis and SEM (Scanning Electron Microscopy) now offer promising alternatives for directly evaluating hair porosity. Scanning Electron Microscopy (SEM) is a technique that uses ionizing radiation to generate images of surfaces. Although the technique requires a high vacuum and coating of the hair sample, it is much more cost-effective than X-ray microtomography, SAXS, or BET. SEM is a widely used technique, and many laboratories are equipped with the necessary instruments and offer analyses at an affordable price. With this technique, it is possible to obtain high-resolution, highly magnified images, which allows for direct visualization of hair pores when the strand is cut transversely. Some microscopes even come with software that enables direct pore measurement from the image; however, this software is sold separately and is usually expensive, often not justifying the investment. The only drawback of using SEM to measure porosity is that it assumes the distribution of pores is the same throughout the hair strand, which is not always true [11]. However, this limitation can be reduced by performing evaluations at multiple points along the same strand. On this context, this study aimed to develop a reliable, cost-effective methodology to measure hair porosity by quantifying the number and size of internal pores using SEM cross-sectional images. The approach involved two phases. In the first phase, we optimized the preparation of hair cross-sections by testing combinations of embedding media and cutting tools to preserve internal hair morphology. Three embedding media and three cutting tools were evaluated. In the second phase, the methodology was validated for reproducibility and sensitivity to cosmetic treatments. Four groups of Caucasian hair swatches were used: one untreated control and three double-bleached groups treated with different products. Cross-sections of these samples were analyzed via SEM, and images were processed using a plugin developed during phase one. This plugin quantified the number and area of internal pores.

RESUMO Danos capilares referem-se ao enfraquecimento e deterioração da estrutura capilar, levando a problemas como quebra, pontas duplas e aspereza da superfície. Esses danos podem afetar várias partes do cabelo e são causados por fatores como má nutrição, tratamentos químicos, cuidados excessivos e estresse ambiental. Entre eles, a descoloração é um dos processos mais agressivos, pois destrói a melanina e, no processo, cliva as ligações dissulfeto que são componentes essenciais da estrutura capilar. Portanto, entender os locais exatos onde ocorrem os danos é importante para o desenvolvimento de produtos cosméticos que visam reparar o cabelo. Para entender e quantificar melhor a deterioração causada na fibra capilar, neste estudo foi empregado um corante catiônico fluorescente como marcador de danos, permitindo uma avaliação precisa dos danos induzidos pelo processo de descoloração desde raiz dos fios até as pontas. A técnica, conhecida como Mapeamento de Danos, utiliza um software para sobrepor resultados de intensidade de fluorescência em imagens de cabelo, produzindo uma representação visual codificada por cores dos níveis de danos. Como esse método foi desenvolvido recentemente pelos autores, uma metodologia adicional bem conhecida, microscopia eletrônica de varredura (MEV), foi usada para examinar os danos superficiais causados por duas fórmulas diferentes de pó descolorante. O nível de dano causado pelos tratamentos descoloridos foi comparado estatisticamente entre eles e em comparação, com um grupo de cabelos não descoloridos do mesmo lote. A correlação entre o mapeamento de fluorescência e os resultados do SEM foi significativa, demonstrando a confiabilidade dessas metodologias na avaliação de danos capilares. Essa abordagem combinada fornece uma maneira abrangente de avaliar os efeitos da descoloração em diferentes pontos ao longo do comprimento do cabelo e apoia o desenvolvimento de produtos cosméticos que visam prevenir ou reduzir os danos capilares seletivamente, nos locais onde é necessário. Palavras-chave: Mapa de Danos; Descoloração; MEV.

Introduction: The cosmetic use of hydrogen peroxide can lead to degradation of protein chains at the hair fiber matrix level due to oxidative effects on –S-S– group that compose human hair. Also, accumulation of H₂O₂ can degrade melanin and damage melanocytes. This study aimed to evaluate the degradation caused by H₂O₂ in hair fiber and follicles using (FTIR-ATR), combined with hair fiber mechanical resistance testing and immunolabeling of melanogenesis associated proteins. Methods: Natural Asian, Caucasian, and Curly hair strands were exposed 1 hour to 3.5% H₂O₂ Young’s modulus and Force at 20% deformation were measured, cystine/amid area ratio and disulfide bridges were quantified from FTIR-ATR spectra. Measurements were taken 5 cm above the strand section submerged in peroxide, highlighting the agent's capillary action and damage displacement. Additionally, follicles obtained from microdissection were cultured and exposed to 3.5% H₂O₂, followed by histological analysis and CKIT and MITF immunolabeling. Results: Hair strands exposed to peroxide damage showed a reduction in Young’s modulus and Force at 20% specific deformation compared to natural hair, following an increasing order of damage: Caucasian, Asian, and Curly. The average cystine/amid area ratio of damaged strands was higher than that of natural hair. Similarly, the area of disulfide bonds in exposed hair strands showed a decrease compared to natural hair, following the same increasing order of damage. Additionally, in the follicles, H₂O₂ exposure caused reductions in MITF and C-kit markings compared to the unexposed group. Discussion and Conclusion: The cystine/amid area ratio reflects the oxidation level of hair disulfide bonds, with a higher ratio indicating greater fiber damage. Lower disulfide bond area values further confirm bond degradation. This highlights that peroxide-induced damage extends beyond the immersed hair portion, potentially reaching follicles and the hair bulb. This finding is supported by the reduction in hair strand resistance caused by oxidative damage to the cortical matrix, with an increasing order of damage: Caucasian, Asian, and Curly. Also, the images obtained highlighted a reduction in MITF and C-KIT proteins, affecting melanogenesis by disrupting regulatory points and signaling pathways in melanocytes. This damage may be associated with long-term effects, including premature graying. Key-words: Immunofluorescence, FTIR-ATR, Young’s modulus, Hair Force, Follicles culture.

Abstract Epidermal proliferation is a natural process that can be accelerated by skin injury. This study aimed to assess the epidermal proliferation induced by glycolic acid, correlating results obtained by fluorescence spectroscopy and micro-image analysis of the skin. Subjects were instructed to apply 5% glycolic acid (GA5%) to their forearms for 21 alternate days. The increase in cell proliferation induced by GA5% was assessed using the 295 nm excitation band (tryptophan). Intensity of desquamation and skin smoothness were assessed using Visioscan®. After 7, 14 and 21 days, the intensity of tryptophan fluorescence from untreated skin remained unchanged, whereas the fluorescence intensity measured from sites treated with GA5% increased. After 3, 7, 14 and 21 days, a trend towards reduced skin smoothness was observed, while desquamation showed a significant increase after 7 days of application of GA5%. Both parameters reached the highest distinction after 7 days. A correlation was observed between intensity of tryptophan fluorescence, desquamation, and skin smoothness. The combination of the three techniques - spectroscopy, desquamation, and smoothness analysis - has proven to be an effective tool for understanding the action mechanism of products designed for cutaneous exfoliation and cell turnover. Keywords: tryptophan fluorescence, cell turnover, epidermal proliferation, desquamation, skin smoothness.

Abstract The degradation of protein chains or the repair obtained by cosmetic treatments in the face of the oxidative effects suffered by the hair fiber, can be evaluated by the behavior of the groups –S-S- and SO3-, that make up human hair. Combability is an attribute measurable through the total energy expended due to friction between the comb and the external surface of the hair tress. This work aimed to evaluate the repair attribute of oxidative damage, using Infrared Spectroscopy, FTIR-ATR (Fourier Transform Infrared Spectroscopy using Attenuated Total Reflectance) combined with the wet and dry hair combing technique. Key-words: Hair, Hair Oxidative process, Repair Factor for Oxidative Damage, FTIR-ATR (Fourier Transform Infrared Spectroscopy using Attenuated Total Reflectance), Combability.

Abstract Hair is a complex polymer that can absorb liquids and small particles through its structure. A complex net of interconnected microvoids formed mainly in the amorphous domain of the polymer. Different types of damage such as chemical treatments, thermal treatments, and others can degrade the amorphous phase increasing the amount of free space. The study of the reduction migration of liquids and small particles is the high interest to the cosmetic industry as the amount of microvoids is related to the hair’s mechanical properties. In this study, we presented a methodology to study the effect of cosmetic products on porosity reduction. Indian Hair bleached twice, were treated with commercial cosmetic treatments (3 swatches per treatment) and then, the swatches were held in a straight position with the tip immersed in an aqueous solution of dye red 80 for 30min. After this time, the distance traveled by the dye was measured. All the treatments presented a lower distance of dye permeation in comparison to the control group. Among the products, the one that promises to repair disulfide bonds presents the highest reduction. The statistical analysis shows that the methodology was reproducible, was able to differentiate treatments, and this differentiation was maintained on the 2 experimental days. Keywords: Hair Permeation, Hair Porosity, Hair Evaluation, Hair Integrity.

Abstract Fiber protein loss is directly related to hair damage processes, such as brushing, dyeing, bleaching, among others. The more damaged the hair fiber, the greater the amount of protein lost from the hair. Natural and double bleached Caucasian, Asian and Curly hair tresses were evaluated on the study. A statistically significant protein loss was observed in hair damaged by double bleached in relation to each respective natural condition, in the 3 types of hair evaluated. Also, a reduction in the enthalpy of keratin degradation for Caucasian, Asian and Curly hair tresses. Thus, the new methodology used was able to measure with statistical significance the protein loss of tresses submitted to oxidative damage from double bleached. Data was corroborated by the Differential Scanning Calorimetry (DSC) technique already established in the evaluation of degradation of the protein constituent of the hair.

O envelhecimento cutâneo representa um processo multifatorial que tem como mecanismo primordial a produção excessiva de radicais livres, caracterizando um ambiente de estresse oxidativo após exposição da pele às diversas agressões externas. O objetivo desse estudo foi avaliar os efeitos de um fotoprotetor (DC-23017-65C) na proteção contra os danos causados pela radiação infravermelha A (IV-A) por meio da quantificação de metaloproteinase de matriz-1 (MMP-1), na ação antipoluição pela quantificação do receptor aril hidrocarboneto (AhR) e na redução da produção de espécies reativas de oxigênio (ROS) induzidas pela radiação UV, utilizando modelo experimental de cultura de células e pele humanas. DC-23017-65C foi aplicado em cultura de pele humana ex vivo, para posterior exposição à radiação UV e fumaça de cigarro, e quantificação de ROS e AhR, respectivamente. Para avaliar a produção de MMP-1 o produto foi aplicado em cultura de fibroblastos e expostos à radiação infravermelha-A (IR-A). Os resultados indicam que DC-23017-65C exerce um efeito profilático contra o aumento da síntese de MMP-1 induzida pela radiação IV-A, preservando a estrutura da matriz extracelular, particularmente o colágeno e atenuando o processo de envelhecimento cutâneo. Além disso, observou-se uma redução significativa na translocação nuclear do receptor AhR e com isso o controle da transcrição de genes envolvidos no estresse oxidativo, inflamação, imunossupressão, pigmentação, entre outros, sugerindo um papel protetor contra os efeitos adversos dos poluentes xenobióticos, o que confere ao produto uma função antipoluição eficaz. Adicionalmente, verificou-se uma diminuição do estresse oxidativo nas culturas pré-tratadas com DC-23017-65C, evidenciando seu efeito protetor contra o aumento excessivo da síntese de ROS, induzido pela radiação UV. Esses achados indicam que o produto DC-23017-65C exerce um efeito abrangente, atenuando os efeitos da exposição à diferentes agentes extrínsecos, como radiação UV, IV-A e poluição. Dessa forma, além da proteção física e química do fotoprotetor, o produto atenua a produção excessiva de biomarcadores que culminam no aumento da susceptibilidade do tecido e evidenciam as alterações inestéticas associadas ao envelhecimento cutâneo. Palavras-chave:Poluição, Infravermelho-A, Ex-vivo, Fotoproteção

The skin barrier relies on key proteins like filaggrin, involucrin, loricrin and keratins for stratum corneum formation, improve cell stability and create a protective shield that ensures skin resilience against external threats. Dysfunction in these proteins can compromise the skin barrier, leading to various dermatological issues The purpose for this study was to evaluate the preclinical effects of a foot-care product 202106059 (FC202106059) on the production of biological markers involved in skin barrier integrity and epidermal repair through immunofluorescence analysis in skin explants.

Skin homeostasis requires a balance between free radical production and natural antioxidant defense. MMPs Collagen breakdown Francisqueti-Ferron FV, Ferron AJT, Garcia JL, et al. Int J Mol Sci. 2019;20(13):3208. • Oxidative process is dynamic and rapid restoration mechanisms are necessary to maintain the structural integrity of the skin. • NRF2 (Nuclear Factor Erythroid 2-related factor 2) is considered the key regulator antioxidant response, being responsible for induce the expression of genes encoding proteins and antioxidant enzymes, playing an important mechanism for cell protection and survival. • Among NRF2 target genes are superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx).

RESUMO O brilho e a cor dos cabelos estão entre os atributos mais importantes de eficiência de produtos cosméticos percebidos pelo consumidor. Foram avaliados três tipos de mechas de cabelos: caucasianos naturais, duplamente descoloridas e mechas tingidas e submetidas a 30 lavagens. Mediu-se a reflexão especular e a espectrometria de cor basal e após dois tipos diferentes de tratamento com produtos cosméticos: linha completa (shampoo, condicionador e máscara) e produtos sem enxague (creme para pentear e óleo). Para as medidas de cor utiliza-se o espectrofotômetro Byk-Gardner Spectro-Guide Sphere Gloss spectrophotometer e sistema de cor CIE-L*a*b* (CIE94) e para as medidas de brilho é utilizado o equipamento Glossmeter (BYK Gardner®) com ângulo de incidência fixo de 85°. Após análise estatística dos valores de brilho Basais e Finais, calculou-se o Fator de Realce da cor dos cabelos através da interpolação dos valores médios de brilho e o parâmetro de cor, pôde-se comprovar a melhora proporcionada pela aplicação de todos os tratamentos cosméticos em todos os tipos de cabelos testados. Sendo observado a maior variação significativa do Fator de Realce dos cabelos principalmente em cabelos que sofreram agressões (duplamente descoloridos e os tingidos e submetidos a 30 lavagens sucessivas com shampoo sem agente condicionantes) quando tratados com produtos não rinsáveis. Quanto maior o valor obtido para o Fator de realce da cor dos cabelos, mais radiante/ intensa a cor dos cabelos após o tratamento cosmético. Um aumento significativo nesses atributos indica uma intensificação da cor e do brilho, deixando-os esteticamente mais bonitos. Palavras-chave: Realce da Cor, Cabelos, Reflexão Especular, Colorimetria.