Preclinical evaluation of a cosmetic product ability to promote the strengthening and ungueal repair

Author: Samara Eberlin

Published at: May 23, 2017

Journal of the American Academy of Dermatology, 76(6) Supplement 1, AB201, 2017.
DOI: http://dx.doi.org/10.1016/j.jaad.2017.04.786
Antonio C. Vanzo, Jr, Liliana B. O. Torloni, Renan Lage, Andréia FC Pereira, Maurizio Mercuri, Amanda F. Pereira, Michelle S. Silva, Gustavo Facchini, Samara Eberlin.

The nails are structures formed by keratinized squamous cells produced by the matrix that grow on the nail bed. The hardness of the nail plate and its adherence to the underlying bed are key properties that make them protective appendages due to the presence of different types of keratins. Studies have shown the presence of K10 and K14 throughout the stratified epithelium of the nail unit. In this context, filaggrin has the role of stabilizing the network of intermediate filaments of K6 and K16 in hyperproliferative stages and promoting increased epidermal cohesion. With the purpose of maintaining the health of nails and improving their appearance, the cosmeceutical market studies new strengthening and fortifying formulations that may influence their biological function. Based on the above, the present study evaluates the efficacy of a nail care cosmetic product (NCCP) through preclinical studies in strengthening and repair activity. Human keratinocytes (HaCat) were incubated with three non-cytotoxic concentrations of NCCP for 48 hours for further evaluation of the production of keratins 1, 10, 14, 16, 17, and filaggrin. The in vitro results show that NCCP has a strengthening and repair effect on nails by stimulating the production of filaggrin and keratins 1, 10, 14, 16, and 17 in human keratinocytes, favoring the nail plate hardness and its adherence to the underlying bed.