In vitro effects of infrared-A radiation on the synthesis of MMP-1, catalase, superoxide dismutase and GADD45 alpha protein

Author: Samara Eberlin

Published at: July 05, 2015

Inflammation & Allergy Drug Targets 14(1), 53-59, 2015.
DOI: http://dx.doi.org/10.2174/1871528114666151022145655
Costa A, Eberlin S, Clerici SP, Abdalla BMZ.

Harmful influences in the process of photoaging and skin damage are associated with infrared A (IRA) radiation, such as disturbance of dermal extracellular matrix by upregulation of matrix metalloproteinase-1 (MMP-1). Furthermore, DNA damage, induction of cytotoxicity, and oxidative stress by decreasing natural antioxidant ability have been reported after acute exposure to IRA. The present study provides additional evidence that IRA radiation response in human skin fibroblasts produces deleterious effects to the cell, such as accelerating aging and weakening their antioxidant defense mechanism. Human skin fibroblasts were exposed to a non-cytotoxic dose of IRA radiation and cultured for different periods for further collection of cell-free supernatants and lysates, and quantification of MMP-1, catalase, superoxide dismutase, and GADD45a. Our results corroborate previously published data and strongly indicate a negative impact of IRA radiation on skin physiology by mechanisms involving reduced endogenous antioxidant enzymatic defense, increased MMP-1, and decreased DNA repair process by reducing GADD45a protein in cultured human fibroblasts. From a clinical perspective, IRA radiation acts by mechanisms distinct from those observed in ultraviolet radiation, indicating the need for developing and making available cosmetics for skin care with properties beyond the protection exerted by traditional sunscreens.