Determination of optimal incubation time for the production of matrix metalloproteinase 1 by human dermal fibroblasts after infrared-A radiation

Author: Samara Eberlin

Published at: March 21, 2014

72nd Annual Meeting AAD - American Academy of Dermatology, Denver, USA, March 21-25, 2014.

Eberlin S, Silva MS, Polettini AJ, Coletta LCD, Costa A, Queiroz MLS.

Recent studies demonstrated that photoaging and skin damage are associated with longer wavelengths, in particular near-infrared radiation. Studies have been conducted in order to determine the damage caused by infrared-A (IR-A) radiation, particularly the upregulation of MMP-1, using in vitro and/or in vivo investigations. For the detection of MMP-1, authors used several IR simulator devices and determined increases in MMP-1 levels through gene expression, western blotting, and immunohistochemistry techniques. Nevertheless, no information concerning MMP-1 quantification using ELISA assay has been published. In this study, we validated the optimal incubation time for the release of MMP-1 in culture supernatants by human dermal fibroblasts (HDF) stimulated with IR-A radiation. Our results demonstrated that irradiation with IR-A resulted in a significant upregulation of MMP-1 production, 1.7-fold in relation to the non-irradiated group, only at an incubation time of 72 hours after radiation. After 24 and 48 hours of IR-A radiation, no significant differences were observed when compared to control groups. In this work, we standardized the optimal time for human fibroblast incubation to produce MMP-1 after IR-A radiation. Contrary to previous studies in the literature, where MMP-1 gene expression is observed after 24 hours from IR-A exposure, the production of this protease in supernatants is only detected after 72 hours of incubation.